The Accumulation of Glucose 6-Phosphate from Glucose and Its Effect in an Escherichia coli Mutant Lacking Phosphoglucose Isomerase and Glucose 6-Phosphate Dehydrogenase

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The accumulation of glucose 6-phosphate from glucose and its effect in an Escherichia coli mutant lacking phosphoglucose isomerase and glucose 6-phosphate dehydrogenase.

When presented with glucose, an Escherichia coli mutant lacking both phosphoglucose isomerase and glucose 6-phosphate dehydrogenase accumulated, internally, high concentrations (approximately 50 mM) of glucose 6-phosphate. It also accumulated glucose in polysaccharide form. Other metabolism of glucose, if any, was very restricted. The accumulation of glucose 6-phosphate was associated with inhi...

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Glucose-6-Phosphate Dehydrogenase

Description Glucose-6-Phosphate Dehydrogenase (G6PD) deficiency is the most common human enzyme deficiency that mainly affects the red blood cells (RBCs). A defect in G6PD enzyme leads to the destruction of premature RBCs causing hemolytic anemia because the body can not compensate the destroyed RBCs. Thus, the affected individuals show jaundice (paleness, yellowing of the skin and whites of th...

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Catalytic reactions of phosphoglucose isomerase with cyclic forms of glucose 6-phosphate and fructose 6-phosphate.

Yeast phosphoglucose isomerase is shown to use and produce both the u and the fi anomeric forms of fructofuranose 6-phosphate. Rapid quench techniques show a Z&fold preference in the utilization of the a! anomer over the /3 anomeric form. The previous report that the (Y anomer of glucopyranose-6-P is the preferred substrate form is confirmed. However the /3 anomer is also shown to react at a sl...

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Responses of the central metabolism in Escherichia coli to phosphoglucose isomerase and glucose-6-phosphate dehydrogenase knockouts.

The responses of Escherichia coli central carbon metabolism to knockout mutations in phosphoglucose isomerase and glucose-6-phosphate (G6P) dehydrogenase genes were investigated by using glucose- and ammonia-limited chemostats. The metabolic network structures and intracellular carbon fluxes in the wild type and in the knockout mutants were characterized by using the complementary methods of fl...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1968

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(18)93160-1